Chloroplast Glutathione Reductase

Pea chloroplast glutathione reductase: purification and characterization.

Glutathione reductase (EC 1.6.4.2) was purified from intact pea (Pisum sativum) chloroplasts by a method which includes affinity chromatography on ADP-agarose. Fractions from the affinity column which had glutathione reductase activity consisted of polypeptides of 60 and 32 kilodaltons. Separation of the proteins by electrophoresis on native gels showed that glutathione reductase activity was a...

Effect of water stress on the chloroplast antioxidant system: I. Alterations in glutathione reductase activity.

The effect of water stress on glutathione reductase and catalase activities was evaluated in leaf blades of field-grown winter wheat (Triticum aestivum L.). Wheat was sown at two seeding rates under both irrigated and dryland conditions. Flag leaves from dryland plants sown at 60 kilograms/hectare showed no change in either glutathione reductase or catalase activities per unit leaf area, while ...

Tobacco chloroplast transformants expressing genes encoding dehydroascorbate reductase, glutathione reductase, and glutathione-S-transferase, exhibit altered anti-oxidant metabolism and improved abiotic stress tolerance.

One approach to understanding the Reactive Oxygen Species (ROS)-scavenging systems in plant stress tolerance is to manipulate the levels of antioxidant enzyme activities. In this study, we expressed in the chloroplast three such enzymes: dehydroascorbate reductase (DHAR), glutathione-S-transferase (GST) and glutathione reductase (GR). Homoplasmic chloroplast transformants containing either DHAR...

Mammalian Selenoprotein Thioredoxin-glutathione Reductase

Molecular basis of glutathione reductase deficiency in human blood cells Short title: Human glutathione reductase deficiency

NMK performed research, collected and analyzed data and wrote the paper; RvZ collected patient material, performed research and analyzed data; MdB performed sequence analysis and analyzed data; GM performed sequence analysis; HV performed patient RBC analysis; NB treated one patient and collected data; CL treated one patient, collected data and wrote a clinical report; KMD interviewed three pat...